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	Provedor de dados BJMBR (5) Autor Brum,M.C.S. (5) Flores,E.F. (5) Weiblen,R. (5) Chowdhury,S.I. (2) Anziliero,D. (1) Arenhart,S. (1) Botton,S.A. (1) Mayer,S.V. (1) Quadros,V.L. (1) Silva,S.C. (1) Vogel,F.S.F. (1) Weiss,M. (1) da-Silva,A.M. (1) Mais... Palavra-chave Bovine viral diarrhea virus (2) Vaccine candidate (2) Antigenic diversity (1) BVDV (1) BoHV-5 (1) Bovine herpesvirus 5 (1) Cattle pathogen (1) Control (1) CpBVDV (1) Cross-neutralization (1) Cytopathology (1) Deletion mutants (1) Differential vaccine (1) GE and TK deletion mutants (1) Glycoprotein E (1) Immunization (1) Monoclonal antibodies (1) NS3 gene (1) Pathogenesis (1) RNA processing (1) Mais... Tipo do documento Info:eu-repo/semantics/article (5) Ano 2015 (1) 2010 (2) 2006 (1) 1998 (1) País Brazil (5) Idioma Inglês (5)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 5 Primeira ... 1 ... Última A bovine herpesvirus 5 recombinant defective in the thymidine kinase (TK) gene and a double mutant lacking TK and the glycoprotein E gene are fully attenuated for rabbits BJMBR Silva,S.C.; Brum,M.C.S.; Weiblen,R.; Flores,E.F.; Chowdhury,S.I.. Bovine herpesvirus 5 (BoHV-5), the agent of herpetic meningoencephalitis in cattle, is an important pathogen of cattle in South America and several efforts have been made to produce safer and more effective vaccines. In the present study, we investigated in rabbits the virulence of three recombinant viruses constructed from a neurovirulent Brazilian BoHV-5 strain (SV507/99). The recombinants are defective in glycoprotein E (BoHV-5gEΔ), thymidine kinase (BoHV-5TKΔ) and both proteins (BoHV-5gEΔTKΔ). Rabbits inoculated with the parental virus (N = 8) developed neurological disease and died or were euthanized in extremis between days 7 and 13 post-infection (pi). Infectivity was detected in several areas of their brains. Three of 8 rabbits inoculated with the... Tipo: Info:eu-repo/semantics/article Palavras-chave: BoHV-5; GE and TK deletion mutants; Pathogenesis; Virulence; Vaccine candidate. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200005 A glycoprotein E gene-deleted bovine herpesvirus 1 as a candidate vaccine strain BJMBR Weiss,M.; Brum,M.C.S.; Anziliero,D.; Weiblen,R.; Flores,E.F.. A bovine herpesvirus 1 (BoHV-1) defective in glycoprotein E (gE) was constructed from a Brazilian genital BoHV-1 isolate, by replacing the full gE coding region with the green fluorescent protein (GFP) gene for selection. Upon co-transfection of MDBK cells with genomic viral DNA plus the GFP-bearing gE-deletion plasmid, three fluorescent recombinant clones were obtained out of approximately 5000 viral plaques. Deletion of the gE gene and the presence of the GFP marker in the genome of recombinant viruses were confirmed by PCR. Despite forming smaller plaques, the BoHV-1△gE recombinants replicated in MDBK cells with similar kinetics and to similar titers to that of the parental virus (SV56/90), demonstrating that the gE deletion had no deleterious effects... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle pathogen; Immunization; Control; Differential vaccine. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900843 A search for RNA insertions and NS3 gene duplication in the genome of cytopathic isolates of bovine viral diarrhea virus BJMBR Quadros,V.L.; Mayer,S.V.; Vogel,F.S.F.; Weiblen,R.; Brum,M.C.S.; Arenhart,S.; Flores,E.F.. Calves born persistently infected with non-cytopathic bovine viral diarrhea virus (ncpBVDV) frequently develop a fatal gastroenteric illness called mucosal disease. Both the original virus (ncpBVDV) and an antigenically identical but cytopathic virus (cpBVDV) can be isolated from animals affected by mucosal disease. Cytopathic BVDVs originate from their ncp counterparts by diverse genetic mechanisms, all leading to the expression of the non-structural polypeptide NS3 as a discrete protein. In contrast, ncpBVDVs express only the large precursor polypeptide, NS2-3, which contains the NS3 sequence within its carboxy-terminal half. We report here the investigation of the mechanism leading to NS3 expression in 41 cpBVDV isolates. An RT-PCR strategy was employed... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine viral diarrhea virus; CpBVDV; NS3 gene; Cytopathology; RNA processing. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700012 Antigenic characterization of Brazilian bovine viral diarrhea virus isolates by monoclonal antibodies and cross-neutralization BJMBR Botton,S.A.; da-Silva,A.M.; Brum,M.C.S.; Weiblen,R.; Flores,E.F.. Nineteen Brazilian isolates of bovine viral diarrhea virus (BVDV) were characterized antigenically with a panel of 19 monoclonal antibodies (mAbs) (Corapi WV, Donis RO and Dubovi EJ (1990) American Journal of Veterinary Research, 55: 1388-1394). Eight isolates were further characterized by cross-neutralization using sheep monospecific antisera. Analysis of mAb binding to viral antigens by indirect immunofluorescence revealed distinct patterns of reactivity among the native viruses. Local isolates differed from the prototype Singer strain in recognition by up to 14 mAbs. Only two mAbs - one to the non-structural protein NS23/p125 and another to the envelope glycoprotein E0/gp48 - recognized 100% of the isolates. No isolate was recognized by more than 14... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine viral diarrhea virus; BVDV; Monoclonal antibodies; Cross-neutralization; Antigenic diversity. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100011 Construction and growth properties of bovine herpesvirus type 5 recombinants defective in the glycoprotein E or thymidine kinase gene or both BJMBR Brum,M.C.S.; Weiblen,R.; Flores,E.F.; Chowdhury,S.I.. Bovine herpesvirus type 5 (BoHV-5) is an important pathogen of cattle in South America. We describe here the construction and characterization of deletion mutants defective in the glycoprotein E (gE) or thymidine kinase (TK) gene or both (gE/TK) from a highly neurovirulent and well-characterized Brazilian BoHV-5 strain (SV507/99). A gE-deleted recombinant virus (BoHV-5 gE∆) was first generated in which the entire gE open reading frame was replaced with a chimeric green fluorescent protein gene. A TK-deleted recombinant virus (BoHV-5 TK∆) was then generated in which most of the TK open reading frame sequences were deleted and replaced with a chimeric β-galactosidase gene. Subsequently, using the BoHV-5 gE∆ virus as backbone, a double gene-deleted (TK plus... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine herpesvirus 5; Glycoprotein E; Thymidine kinase; Deletion mutants; Vaccine candidate; Recombinant herpesvirus. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200014 Registros recuperados: 5 Primeira ... 1 ... Última

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